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KMID : 0378119940210010067
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Chungnam Medical Journal
1994 Volume.21 No. 1 p.67 ~ p.76
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Detection of Pneumocystis carinii DNA From Bronchial Lavage Fluid of Cortisonized Rats Using a Polymerase Chain Reaction : A pilot study
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Kim Ju-Ock
Hong Seok-Cheol
Han Pyo-Seong
Lee Jong-Jin
Kim Sun-Young
Lee Young-Ha
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Abstract
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Background : Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study, @ES
Methods : @EN PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method.
Results : @EN Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7).
Conclusion : @EN This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully induced on cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive method for PC DNA detection.
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